![]() Potato patatin is considered a valuable plant protein by the food industry for to its exceptional functional properties and nutritional value. Apart from studying the swelling characteristics of the microgel shells in dependence of temperature, we provide data from 2- and 3-dimensional assemblies demonstrating the suitability for structural analysis using optical microscopy and laser diffraction. In this work, we report on the one-pot synthesis of silica-poly(N-isopropylacrylamide) core-shell microgels with overall dimensions reaching the micrometer range and excellent control over the shell-to-core size ratio. Surprisingly robust, yet versatile protocols that yield micrometer sized core-shell microgels with rigid cores and soft deformable shells suitable for analysis by optical light microscopy and/or laser diffraction are rarely available. Consequently, assembly studies relied on electron microscopy/atomic force microscopy and neutron/X-ray scattering methods to resolve the relevant length scales required for the microstructure analysis. In the last years, most synthesis approaches reported focused on sub-micrometer total particle dimensions. The interaction and assembly behavior of such core-shell microgels strongly depend on the shell-to-core size ratio and the overall particle size. Seeded precipitation polymerization allows the encapsulation of a plethora of inorganic and organic (nano)particles by thick, soft and deformable microgel shells. Furthermore, the nanoparticles displayed good cytotoxicity against L929 fibroblast and were found to possess strong antimicrobial properties, inhibiting the growth of S. Also, GVE-SNP exhibited a significant and dose-response inhibitory effect against tyrosinase. The nanoparticles displayed potent radical scavenging activity against ABTṠ⁺ and DPPḢ. The FTIR and RAMAN spectra indicated that GVE-SNP surface was properly capped by bioactives from GVE. GVE-SNP was found to present stable, well-dispersed, round, uniform, and crystalline nanoparticles of about 5.88 nm. The results revealed that successful synthesis of GVE-SNP was instantaneous and maximum intensity of the plasmonic peak at 425 nm was achieved in less than 10 min. This study presents a comprehensive delineation of the sustainable phyto-fabrication of biogenic guava phenolic extract functionalized silver nanoparticles (GVE-SNP) based on guava phenolic extract as the sole reductant/stabilizer, as well as the synthesis optimization, thorough physicochemical characterization and potential biological applications of the as-prepared nanosilver. There had been some reports demonstrating the green synthesis of silver nanoparticles using guava (Psidium guajava (L.) extract) however, detailed and in-depth interrogation of the vital synthesis parameters for rapid, facile, efficacious synthesis at room temperature, and robust characterization of the as-prepared nanoparticle is currently lacking. Pitfalls and subsequent development of the best reproducible method.Use of the capillary method to characterize high value samples in the lowest, reproducible volume.We demonstrate the statistically identical characterisation of the new scheme and dipping against the reference measurements, but in sample volumes reduced by 1 and 3 orders of magnitude, respectively, key for high value applications such as pharmaceutical development where sample costs of $100 k per mg are common and in the environmental & medical sciences where samples may be difficult or unethical to collect in bulk. In this work, we carefully validate capillary dipping and sealing with a clay plug for DLS against reference measurements in a high quality 1 cm cuvette and then introduce a simple capillary loading scheme that reproducibly places a >3 μl sample in the correct location for a DLS measurement. Sample loading for other capillary-based modalities, such as blood analysis, is typically achieved by dipping the capillary into the bulk sample, however, DLS is exquisitely sensitive to static scattering such as from a fluid meniscus or sample dried on the outside of the capillary and is sometimes used for extended measurement times where evaporation must be avoided. Capillary dynamic light scattering (DLS) is a new, simple and enabling technique, that increases the size range of DLS by over an order of magnitude in a cheap, disposable, but high optical quality, glass capillary.
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